Chelsea assistant first team coach Steve Holland says midfielder Ramires will not feature against Bolton on Wednesday.
The 27-year-old was replaced by Andre Schurrle during the Manchester City game and is expected to be assessed in the coming days by the Chelsea medical team.
"Ramires had a problem in his abductor muscle in the game which resulted in him being substituted in the second half," Holland said during his press conference.
"It is a minor injury but it will keep him out of the game against Bolton and we will review him in the days following that."
Other players are expected to be rotated for tomorrow's game with Holland saying it is unclear who will be in the starting line-up.
"We haven't finalised the squad for tomorrow yet. We have looked at the players following the game against Manchester City and it is an opportunity to rotate, we will do that for sure, but in terms of who is playing and who isn't, that is unclear," added Holland.
"For the first weeks of the season we have had one game a week and the international break.
"Now the two games-a-week cycle kicks in and it is important you have a pool of players who are ready to handle that schedule.
"Last week was the first European matches and there were four English clubs in the Champions League and two in the Europa League, and only one managed to be unbeaten in the games before and after the European game and in the European game, so it is that time of the season when it is demanding and we are very fortunate to have great depth in quality.
"We have a small group of players who I am sure have been frustrated not to have played more and will possibly have the opportunity to show what they can do tomorrow."
